Macromolecular composition of phloem exudate from white lupin (Lupinus albus L.).

ABSTRACT:

Background

Members of the legume genus Lupinus exude phloem 'spontaneously' from incisions made to the vasculature. This feature was exploited to document macromolecules present in exudate of white lupin (Lupinus albus [L.] cv Kiev mutant), in particular to identify proteins and RNA molecules, including microRNA (miRNA).

Results

Proteomic analysis tentatively identified 86 proteins from 130 spots collected from 2D gels analysed by partial amino acid sequence determination using MS/MS. Analysis of a cDNA library constructed from exudate identified 609 unique transcripts. Both proteins and transcripts were classified into functional groups. The largest group of proteins comprised those involved in metabolism (24%), followed by protein modification/turnover (9%), redox regulati

SUBMITTER: Rodriguez-Medina C 

PROVIDER: S-EPMC3055823 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature