High-throughput 454 resequencing for allele discovery and recombination mapping in Plasmodium falciparum.

ABSTRACT:

Background

Knowledge of the origins, distribution, and inheritance of variation in the malaria parasite (Plasmodium falciparum) genome is crucial for understanding its evolution; however the 81% (A+T) genome poses challenges to high-throughput sequencing technologies. We explore the viability of the Roche 454 Genome Sequencer FLX (GS FLX) high throughput sequencing technology for both whole genome sequencing and fine-resolution characterization of genetic exchange in malaria parasites.

Results

We present a scheme to survey recombination in the haploid stage genomes of two sibling parasite clones, using whole genome pyrosequencing that includes a sliding window approach to predict recombination breakpoints. Whole genome shotgun (WGS) sequencing generated approximately 2 milli

SUBMITTER: Samarakoon U 

PROVIDER: S-EPMC3055840 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature