Viral metagenome analysis to guide human pathogen monitoring in environmental samples.

ABSTRACT:

Aims

The aim of this study was to develop and demonstrate an approach for describing the diversity of human pathogenic viruses in an environmentally isolated viral metagenome.

Methods and results

In silico bioinformatic experiments were used to select an optimum annotation strategy for discovering human viruses in virome data sets and applied to annotate a class B biosolid virome. Results from the in silico study indicated that <1% errors in virus identification could be achieved when nucleotide-based search programs (BLASTn or tBLASTx), viral genome only databases and sequence reads >200 nt were considered. Within the 51,925 annotated sequences, 94 DNA and 19 RNA sequences were identified as human viruses. Virus diversity included environmentally transmitted agents such as

SUBMITTER: Bibby K 

PROVIDER: S-EPMC3055918 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature