Deciphering modifications in swine cardiac troponin I by top-down high-resolution tandem mass spectrometry.

ABSTRACT: Cardiac troponin I (cTnI) is an important regulatory protein in cardiac muscle, and its modification represents a key mechanism in the regulation of cardiac muscle contraction and relaxation. cTnI is often referred to as the "gold-standard" serum biomarker for diagnosing patients with acute cardiac injury since it is unique to the heart and released into the circulation following necrotic death of cardiac tissue. The swine (Sus scrofa) heart model is extremely valuable for cardiovascular research since the heart anatomy and coronary artery distribution of swine are almost identical to those of humans. Herein, we report a comprehensive characterization of the modifications in swine cTnI using top-down high-resolution tandem mass spectrometry in conjugation with immunoaffinity chromatography

SUBMITTER: Zhang J 

PROVIDER: S-EPMC3056346 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature