Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli.

ABSTRACT:

Background

Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expression requires choosing from a vast number of possible DNA sequences and different codon optimization methods have been used in the past decade. Here, a comparative study of the two most common methods is presented using calf prochymosin as a model.

Results

Seven sequences encoding calf prochymosin have been designed, two using the "one amino acid-one codon" method and five using a "codon randomization" strategy. When expressed in Escherichia coli, the variants op

SUBMITTER: Menzella HG 

PROVIDER: S-EPMC3056764 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature