Antibody-directed lentiviral gene transduction in early immature hematopoietic progenitor cells.

ABSTRACT:

Background

The specific and efficient transduction of retroviral particles remains problematic for in vivo and ex vivo gene therapy studies, where the targeting cell population is a heterogeneous bulk population.

Methods

Pseudotyping lentiviral particles with Sindbis virus envelope (Env) proteins modified with an immunoglobulin Fc-binding domain presents a method of selecting cells within a mixed population through antibody (Ab)-mediated targeting. Conditions were tested for targeted lentiviral gene delivery to hematopoietic progenitor cells via Ab-conjugated envelopes independent of CD34.

Results

Conditions to optimize the efficiency of gene delivery were established using the ABCG2 multidrug resistance protein, associated with stem cell phenotypes, as the cell surfa

SUBMITTER: Zhang X 

PROVIDER: S-EPMC3057560 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature