Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae.

ABSTRACT: Previous studies suggested that the zinc-responsive Zap1 transcriptional activator directly regulates the expression of over 80 genes in Saccharomyces cerevisiae. Many of these genes play key roles to enhance the ability of yeast cells to grow under zinc-limiting conditions. Zap1 is unusual among transcriptional activators in that it contains two activation domains, designated AD1 and AD2, which are regulated independently by zinc. These two domains are evolutionarily conserved among Zap1 orthologs suggesting that they are both important for Zap1 function. In this study, we have examined the roles of AD1 and AD2 in low zinc growth and the regulation of Zap1 target gene expression. Using alleles that are specifically disrupted for either AD1 or AD2 function, we found that these domains are

SUBMITTER: Frey AG 

PROVIDER: S-EPMC3057842 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature