ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth.

ABSTRACT:

Background

Centromere protein A (CENP-A) plays important roles in cell-cycle regulation and genetic stability. Herein, we aimed to investigate its expression pattern, clinical significance, and biological function in hepatocellular carcinoma (HCC).

Methodology/principal findings

CENP-A expression at the mRNA and protein levels was examined in 20 pairs of fresh HCCs and corresponding nontumor liver tissues. Immunohistochemistry for CENP-A was performed on 80 paraffin-embedded HCC specimens, and the clinical significance of its expression was analyzed. A human HCC cell line HepG2 with high abundance of CENP-A was used to study the effects of manipulating CENP-A on HCC growth. Quantitative real-time polymerase chain reaction arrays and Western blot analysis were employed to ide

SUBMITTER: Li Y 

PROVIDER: S-EPMC3058037 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature