Novel high-throughput screen against Candida albicans identifies antifungal potentiators and agents effective against biofilms.

ABSTRACT:

Objectives

Microbial adhesion and biofilms have important implications for human health and disease. Candida albicans is an opportunistic pathogen which forms drug-resistant biofilms that contribute to the recalcitrance of disease. We have developed a high-throughput screen for potentiators of clotrimazole, a common therapy for Candida infections, including vaginitis and thrush. The screen was performed against C. albicans biofilms grown in microtitre plates in order to target the most resilient forms of the pathogen.

Methods

Biofilm growth, in individual wells of 384-well plates, was measured using the metabolic indicator alamarBlue® and found to be very consistent and reproducible. This assay was used to test the effect of more than 120 000 small molecule compounds from th

SUBMITTER: LaFleur MD 

PROVIDER: S-EPMC3058565 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature