Pulsed multiple reaction monitoring approach to enhancing sensitivity of a tandem quadrupole mass spectrometer.

ABSTRACT: Liquid chromatography (LC)-triple quadrupole mass spectrometers operating in a multiple reaction monitoring (MRM) mode are increasingly used for quantitative analysis of low-abundance analytes in highly complex biochemical matrixes. After development and selection of optimum MRM transitions, sensitivity and data quality limitations are largely related to mass spectral peak interferences from sample or matrix constituents and statistical limitations at low number of ions reaching the detector. Herein, we report on a new approach to enhancing MRM sensitivity by converting the continuous stream of ions from the ion source into a pulsed ion beam through the use of an ion funnel trap (IFT). Evaluation of the pulsed MRM approach was performed with a tryptic digest of Shewanella oneidensis strain

SUBMITTER: Belov ME 

PROVIDER: S-EPMC3059105 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature