Phenotypic diversity of cytomegalovirus DNA polymerase gene variants observed after antiviral therapy.

ABSTRACT:

Background

Cytomegalovirus UL54 DNA polymerase mutations observed in clinical specimens are of diagnostic significance if confirmed to affect antiviral drug susceptibility.

Objectives

Validate an updated recombinant phenotyping method to determine the degree of drug resistance conferred by previously uncharacterized UL54 sequence variants, in comparison with known resistance-related mutations.

Study design

Bacterial artificial chromosome clones of viral DNA were mutagenized by recombination, transfected to produce live virus and phenotyped by standardized reporter-based yield reduction assays.

Results

Sixteen recombinant viruses were constructed, representing baseline sequences, known resistance-related mutations and amino acid changes of unproven significance

SUBMITTER: Chou S 

PROVIDER: S-EPMC3059355 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature