Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.

ABSTRACT: Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyrosequencing (TEFAP) analysis amplified sequences from callus homologous to diverse fungal and bacterial taxa. Culturing isolated some seed borne endophyte taxa which could be readily propagated apart from the host. Microbial cells were observed within biofilm-like residues associated with plant cell surfaces and intercellular spaces. Various universal primers amplified both plant and microbial sequences, with different primers revealing different patterns of fungal diversity. Bacterial and fungal TEFA

SUBMITTER: Lucero ME 

PROVIDER: S-EPMC3060086 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature