High throughput nano-liter RT-qPCR to classify soil contamination using a soil arthropod.

ABSTRACT:

Background

To incorporate genomics data into environmental assessments a mechanistic perspective of interactions between chemicals and induced biological processes needs to be developed. Since chemical compounds with structural similarity often induce comparable biological responses in exposed animals, gene expression signatures can serve as a starting point for the assessment of chemicals and their toxicity, but only when relevant and stable gene panels are available. To design such a panel, we isolated differentially expressed gene fragments from the soil arthropod Folsomia candida, a species often used for ecotoxicological testing. Animals were exposed to two chemically distinct compounds, being a metal (cadmium) and a polycyclic aromatic hydrocarbon (phenanthrene). We investiga

SUBMITTER: de Boer ME 

PROVIDER: S-EPMC3060125 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature