Discovery of barley miRNAs through deep sequencing of short reads.

ABSTRACT:

Background

MicroRNAs are important components of the regulatory network of biological systems and thousands have been discovered in both animals and plants. Systematic investigations performed in species with sequenced genomes such as Arabidopsis, rice, poplar and Brachypodium have provided insights into the evolutionary relationships of this class of small RNAs among plants. However, miRNAs from barley, one of the most important cereal crops, remain unknown.

Results

We performed a large scale study of barley miRNAs through deep sequencing of small RNAs extracted from leaves of two barley cultivars. By using the presence of miRNA precursor sequences in related genomes as one of a number of supporting criteria, we identified up to 100 miRNAs in barley. Of these only 56 have o

SUBMITTER: Schreiber AW 

PROVIDER: S-EPMC3060140 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature