Enhancement effects of martentoxin on glioma BK channel and BK channel (α+β1) subtypes.

ABSTRACT:

Background

BK channels are usually activated by membrane depolarization and cytoplasmic Ca(2+). Especially,the activity of BK channel (α+β4) can be modulated by martentoxin, a 37 residues peptide, with Ca(2+)-dependent manner. gBK channel (glioma BK channel) and BK channel (α+β1) possessed higher Ca(2+) sensitivity than other known BK channel subtypes.

Methodology and principal findings

The present study investigated the modulatory characteristics of martentoxin on these two BK channel subtypes by electrophysiological recordings, cell proliferation and Ca(2+) imaging. In the presence of cytoplasmic Ca(2+), martentoxin could enhance the activities of both gBK and BK channel (α+β1) subtypes in dose-dependent manner with EC(50) of 46.7 nM and 495 nM respectively, while not shif

SUBMITTER: Tao J 

PROVIDER: S-EPMC3060806 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature