RNA interference of four genes in adult Bactrocera dorsalis by feeding their dsRNAs.

ABSTRACT:

Background

RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner. Recently silencing the target gene through feeding has been successfully carried out in many insect species.

Methodology/principal findings

Escherichia coli strain HT115 was genetically engineered to express dsRNA targeting genes that encode ribosomal protein Rpl19, V type ATPase D subunit, the fatty acid elongase Noa and a small GTPase Rab11. qRT-PCR showed that mRNA level of four target genes was reduced compared to ds-egfp control by feeding either engineered bacteria or dsRNAs. The maximum down-regulation of each gene varied from 35% to 100%. Tissue specific examination indicated that RNAi could be observed not only in midgut but also in other tissues like th

SUBMITTER: Li X 

PROVIDER: S-EPMC3060817 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature