In vivo functional genomic studies of sterol carrier protein-2 gene in the yellow fever mosquito.

ABSTRACT: A simple and efficient DNA delivery method to introduce extrachromosomal DNA into mosquito embryos would significantly aid functional genomic studies. The conventional method for delivery of DNA into insects is to inject the DNA directly into the embryos. Taking advantage of the unique aspects of mosquito reproductive physiology during vitellogenesis and an in vivo transfection reagent that mediates DNA uptake in cells via endocytosis, we have developed a new method to introduce DNA into mosquito embryos vertically via microinjection of DNA vectors in vitellogenic females without directly manipulating the embryos. Our method was able to introduce inducible gene expression vectors transiently into F0 mosquitoes to perform functional studies in vivo without transgenic lines. The high efficie

SUBMITTER: Peng R 

PROVIDER: S-EPMC3060925 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature