Effects of a recombinant gene expression on ColE1-like plasmid segregation in Escherichia coli.

ABSTRACT:

Background

Segregation of expression plasmids leads to loss of recombinant DNA from transformed bacterial cells due to the irregular distribution of plasmids between the daughter cells during cell division. Under non-selective conditions this segregational instability results in a heterogeneous population of cells, where the non-productive plasmid-free cells overgrow the plasmid-bearing cells thus decreasing the yield of recombinant protein. Amongst the factors affecting segregational plasmid instability are: the plasmid design, plasmid copy-number, host cell genotype, fermentation conditions etc. This study aims to investigate the influence of transcription and translation on the segregation of recombinant plasmids designed for constitutive gene expression in Escherichia coli LE39

SUBMITTER: Popov M 

PROVIDER: S-EPMC3061898 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature