Simultaneous multiple-excitation multiphoton microscopy yields increased imaging sensitivity and specificity.

ABSTRACT:

Background

Multiphoton microscopy (MPM) offers many advantages over conventional wide-field and confocal laser scanning microscopy (CLSM) for imaging biological samples such as 3D resolution of excitation, reduced phototoxicity, and deeper tissue imaging. However, adapting MPM for critical multi-color measurements presents a challenge because of the largely overlapping two-photon absorption (TPA) peaks of common biological fluorophores. Currently, most multi-color MPM relies on the absorbance at one intermediate wavelength of multiple dyes, which introduces problems such as decreased and unequal excitation efficiency across the set of dyes.

Results

Here we describe an MPM system incorporating two, independently controlled sources of two-photon excitation whose wavelengths ar

SUBMITTER: Butko MT 

PROVIDER: S-EPMC3062589 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature