Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus.

ABSTRACT:

Background

Crustacean moulting is a complex process involving many regulatory pathways. A holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study. Custom cDNA microarrays were constructed for Portunus pelagicus. The printed arrays contained 5000 transcripts derived from both the whole organism, and from individual organs such as the brain, eyestalk, mandibular organ and Y-organ from all moult cycle stages.

Results

A total of 556 clones were sequenced from the cDNA libraries used to construct the arrays. These cDNAs represented 175 singletons and 62 contigs, resulting in 237 unique putative genes. The gene sequences were classified into the following biological fu

SUBMITTER: Kuballa AV 

PROVIDER: S-EPMC3062621 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature