DNA ploidy, nuclear size, proliferation index and DNA-hypomethylation in ovarian cancer.

ABSTRACT:

Objective

The present study was undertaken to analyze the impact of epigenetic alterations with a main focus on nuclear area, aneuploidy, hyperploidy, and proliferation in 70 ovarian cancer specimens.

Methods

Morphometric changes and somatic chromosomal ploidy status were assessed by Feulgen spectrophotometry. DNA-hypomethylation of LINE1 repeats was analyzed by means of MethyLight PCR, and methylation levels of satellite 2 (Sat2) and satellite alpha (Satα) DNA sequences in chromosome 1 were measured by Southern blot analysis. These parameters were analyzed with regard to correlations as well as to recurrence and survival.

Results

We identified a significant association between LINE1 DNA-hypomethylation and patient age (p=0.029). Furthermore, LINE1 DNA-hypomethylation

SUBMITTER: Zeimet AG 

PROVIDER: S-EPMC3062717 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature