Regulation of glioblastoma progression by cord blood stem cells is mediated by downregulation of cyclin D1.

ABSTRACT:

Background

The normal progression of the cell cycle requires sequential expression of cyclins. Rapid induction of cyclin D1 and its associated binding with cyclin-dependent kinases, in the presence or absence of mitogenic signals, often is considered a rate-limiting step during cell cycle progression through the G(1) phase.

Methodology/principal findings

In the present study, human umbilical cord blood stem cells (hUCBSC) in co-cultures with glioblastoma cells (U251 and 5310) not only induced G(0)-G(1) phase arrest, but also reduced the number of cells at S and G(2)-M phases of cell cycle. Cell cycle regulatory proteins showed decreased expression levels upon treatment with hUCBSC as revealed by Western and FACS analyses. Inhibition of cyclin D1 activity by hUCBSC treatment

SUBMITTER: Velpula KK 

PROVIDER: S-EPMC3063796 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature