A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus.

ABSTRACT:

Background

Infectious bursal disease (IBD) is a highly contagious immunosuppressive disease in young chickens caused by infectious bursal disease virus (IBDV). It causes huge economic losses to the poultry industry. The objective of this study is to develop a loop-mediated isothermal amplification (LAMP) method for the detection and discrimination of IBDV.

Results

In this study, we applied reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect IBDV in one simple step and further identified the very virulent strain from non-vvIBDVs with a simply post-amplification restriction enzyme analysis. Based on sequence analysis, a set of two inner, two outer and two loop primers were designed to target the VP5 gene and they showed great specificity with no cr

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3063808 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature