Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation.

ABSTRACT: mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.

SUBMITTER: Fu J 

PROVIDER: S-EPMC3064370 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature