Combinatorial guidance by CCR7 ligands for T lymphocytes migration in co-existing chemokine fields.

ABSTRACT: Chemokines mediate the trafficking and positioning of lymphocytes in lymphoid tissues that is crucial for immune surveillance and immune responses. In particular, a CCR7 ligand, CCL21, plays important roles in recruiting T cells to secondary lymphoid tissues (SLT). Furthermore, CCL21 together with another CCR7 ligand, CCL19, direct the navigation and compartmentation of T cells within SLT. However, the distinct roles of these two chemokines for regulating cell trafficking and positioning are not clear. In this study, we explore the effect of co-existing CCL19 and CCL21 concentration fields on guiding T cell migration. Using microfluidic devices that can configure single and superimposed chemokine fields we show that under physiological gradient conditions, human peripheral blood T cells ch

SUBMITTER: Nandagopal S 

PROVIDER: S-EPMC3064588 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature