High-throughput mapping of protein occupancy identifies functional elements without the restriction of a candidate factor approach.

ABSTRACT: There are a variety of in vivo and in vitro methods to determine the genome-wide specificity of a particular trans-acting factor. However there is an inherent limitation to these candidate approaches. Most biological studies focus on the regulation of particular genes, which are bound by numerous unknown trans-acting factors. Therefore, most biological inquiries would be better addressed by a method that maps all trans-acting factors that bind particular regions rather than identifying all regions bound by a particular trans-acting factor. Here, we present a high-throughput binding assay that returns thousands of unbiased measurements of complex formation on nucleic acid. We applied this method to identify transcriptional complexes that form on DNA regions upstream of genes involved in plu

SUBMITTER: Ferraris L 

PROVIDER: S-EPMC3064794 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature