Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coli.

ABSTRACT: Nucleoid-associated proteins (NAPs) are global regulators of gene expression in Escherichia coli, which affect DNA conformation by bending, wrapping and bridging the DNA. Two of these--H-NS and Fis--bind to specific DNA sequences and structures. Because of their importance to global gene expression, the binding of these NAPs to the DNA was previously investigated on a genome-wide scale using ChIP-chip. However, variation in their binding profiles across the growth phase and the genome-scale nature of their impact on gene expression remain poorly understood. Here, we present a genome-scale investigation of H-NS and Fis binding to the E. coli chromosome using chromatin immunoprecipitation combined with high-throughput sequencing (ChIP-seq). By performing our experiments under multiple time-p

SUBMITTER: Kahramanoglou C 

PROVIDER: S-EPMC3064808 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature