Role of glycosylation in structure and stability of Erythrina corallodendron lectin (EcorL): a molecular dynamics study.

ABSTRACT: The effect of glycosylation on structure and stability of glycoproteins has been a topic of considerable interest. In this work, we have investigated the solution conformation of the oligosaccharide and its effect on the structure and stability of the glycoprotein by carrying out a series of long Molecular dynamics (MD) simulations on glycosylated Erythrina corallodendron lectin (EcorL) and nonglycosylated recombinant Erythrina corallodendron lectin (rEcorL). Our results indicate that, despite the similarity in overall three dimensional structures, glycosylated EcorL has lesser nonpolar solvent accessible surface area compared to nonglycosylated EcorL. This might explain the experimental observation of higher thermodynamic stability for glycosylated EcorL compared to nonglycosylated EcorL.

SUBMITTER: Kaushik S 

PROVIDER: S-EPMC3064827 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature