Evaluating sequence-derived mtDNA length heteroplasmy by amplicon size analysis.

ABSTRACT: Length heteroplasmy (LH) in mitochondrial (mt)DNA is usually observed in homopolymeric tracts and manifest as mixture of various length variants. The generally used difference-coded annotation to report mtDNA haplotypes does not express the degree of LH variation present in a sample, even more so, it is sometimes difficult to establish which length variants are present and clearly distinguishable from background noise. It has therefore become routine practice for some researchers to call the dominant type, the "major molecule", which represents the LH variant that is most abundant in a DNA extract. In the majority of cases a clear single dominant variant can be identified. However, in some samples this interpretation is difficult, i.e. when (almost) equally quantitative LH variants are pre

SUBMITTER: Berger C 

PROVIDER: S-EPMC3064993 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature