Cytoplasmic intron sequence-retaining transcripts can be dendritically targeted via ID element retrotransposons.

ABSTRACT: RNA precursors give rise to mRNA after splicing of intronic sequences traditionally thought to occur in the nucleus. Here, we show that intron sequences are retained in a number of dendritically-targeted mRNAs, by using microarray and Illumina sequencing of isolated dendritic mRNA as well as in situ hybridization. Many of the retained introns contain ID elements, a class of SINE retrotransposon. A portion of these SINEs confers dendritic targeting to exogenous and endogenous transcripts showing the necessity of ID-mediated mechanisms for the targeting of different transcripts to dendrites. ID elements are capable of selectively altering the distribution of endogenous proteins, providing a link between intronic SINEs and protein function. As such, the ID element represents a common dendriti

SUBMITTER: Buckley PT 

PROVIDER: S-EPMC3065018 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature