Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy.

ABSTRACT: Conventional histopathology involves sampling, sectioning and staining of tissue specimens prior to microscopic evaluation, and provides diagnostic information at a single location and point in time. In vivo microscopy and molecular-targeted optical labeling are two rapidly developing fields, which together have the potential to provide anatomical and functional indications of disease by staining and imaging tissue in situ. To address the need for high-resolution imaging instrumentation, we have developed a compact, robust, and inexpensive fiber-optic microendoscopy system based around wide-field LED illumination, a flexible 1 mm diameter fiber-optic bundle, and a color CCD camera. We demonstrate the sub-cellular resolution imaging capabilities of the system through a series of experiments

SUBMITTER: Muldoon TJ 

PROVIDER: S-EPMC3065245 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature