Microarrays and RNA-Seq identify molecular mechanisms driving the end of nephron production.

ABSTRACT:

Background

The production of nephrons suddenly ends in mice shortly after birth when the remaining cells of the multi-potent progenitor mesenchyme begin to differentiate into nephrons. We exploited this terminal wave of nephron production using both microarrays and RNA-Seq to serially evaluate gene transcript levels in the progenitors. This strategy allowed us to define the changing gene expression states following induction and the onset of differentiation after birth.

Results

Microarray and RNA-Seq studies of the progenitors detected a change in the expression profiles of several classes of genes early after birth. One functional class, a class of genes associated with cellular proliferation, was activated. Analysis of proliferation with a nucleotide analog demonstrated in

SUBMITTER: Brunskill EW 

PROVIDER: S-EPMC3065427 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature