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Field detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR.

ABSTRACT: A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.

SUBMITTER: Worrell C 

PROVIDER: S-EPMC3067307 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Field detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR.

Worrell Caitlin C   Xiao Ning N   Vidal Jorge E JE   Chen Lin L   Zhong Bo B   Remais Justin J  

Applied and environmental microbiology 20110128 6

A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria. ...[more]

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