[NiFe] hydrogenase from Alteromonas macleodii with unusual stability in the presence of oxygen and high temperature.

ABSTRACT: Hydrogenases are enzymes involved in the bioproduction of hydrogen, a clean alternative energy source whose combustion generates water as the only end product. In this article we identified and characterized a [NiFe] hydrogenase from the marine bacterium Alteromonas macleodii "deep ecotype" with unusual stability toward oxygen and high temperature. The A. macleodii hydrogenase (HynSL) can catalyze both H(2) evolution and H(2) uptake reactions. HynSL was expressed in A. macleodii under aerobic conditions and reached the maximum activity when the cells entered the late exponential phase. The higher level of hydrogenase activity was accompanied by a greater abundance of the HynSL protein in the late-log or stationary phase. The addition of nickel to the growth medium significantly enhanced th

SUBMITTER: Vargas WA 

PROVIDER: S-EPMC3067314 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature