Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples.

ABSTRACT: Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis. In this study, the two techniques were compared and contrasted for analysis of the bacterial composition in three fecal and three small intestinal samples from human individuals. As PCR remains a crucial step in sample preparation for both techniques, different forward primers were used for amplification to assess their impact on microbial profiling results. An average of 7,944 pyrosequences, spanning the V1 and V2 region of 16S rRNA genes, was obtained per sample. Although primer choice in barcoded pyrosequencing did not affect species richness and diversity estimates, dete

SUBMITTER: van den Bogert B 

PROVIDER: S-EPMC3067328 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature