Multiple approaches to enhance the cultivability of bacteria associated with the marine sponge Haliclona (gellius) sp.

ABSTRACT: Three methods were examined to cultivate bacteria associated with the marine sponge Haliclona (gellius) sp.: agar plate cultures, liquid cultures, and floating filter cultures. A variety of oligotrophic media were employed, including media with aqueous and organic sponge extracts, bacterial signal molecules, and siderophores. More than 3,900 isolates were analyzed, and 205 operational taxonomic units (OTUs) were identified. Media containing low concentrations of mucin or a mixture of peptone and starch were most successful for the isolation of diversity, while the commonly used marine broth did not result in a high diversity among isolates. The addition of antibiotics generally led to a reduced diversity on plates but yielded different bacteria than other media. In addition, diversity patt

SUBMITTER: Sipkema D 

PROVIDER: S-EPMC3067343 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature