Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologen.

ABSTRACT: Marker removal strategies were developed for Thermoanaerobacterium saccharolyticum to select against the pyrF gene and the pta and ack genes. The pta- and ack-based haloacetate selective strategy was subsequently used to create strain M0355, a markerless Δldh Δpta Δack strain that produces ethanol at a high yield.

SUBMITTER: Shaw AJ 

PROVIDER: S-EPMC3067457 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature