Catalytic versus inhibitory promiscuity in cytochrome P450s: implications for evolution of new function.

ABSTRACT: Catalytically promiscuous enzymes are intermediates in the evolution of new function from an existing pool of protein scaffolds. However, promiscuity will only confer an evolutionary advantage if other useful properties are not compromised or if there is no "negative trade-off" induced by the mutations that yield promiscuity. Therefore, identification and characterization of negative trade-offs incurred during the emergence of promiscuity are required to further develop the evolutionary models and to optimize in vitro evolution. One potential negative trade-off of catalytic promiscuity is increased susceptibility to inhibition, or inhibitory promiscuity. Here we exploit cytochrome P450s (CYPs) as a model protein scaffold that spans a vast range of catalytic promiscuity and apply a quantita

SUBMITTER: Foti RS 

PROVIDER: S-EPMC3068220 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature