Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma.

ABSTRACT:

Aim

To evaluate the diagnostic values of serum autoantibodies against matrix metalloproteinase-7 (MMP-7) in patients with esophageal squamous cell carcinoma (ESCC).

Methods

The MMP-7 cDNA was cloned from ESCC tissues, and MMP-7 was expressed and purified from a prokaryotic system. MMP-7 autoantibodies were then measured in sera from 50 patients with primary ESCC and 58 risk-matched controls, using a reverse capture enzyme-linked immunosorbent assay (ELISA) in which autoantibodies to MMP-7 bound to the purified MMP-7 proteins. In addition, MMP-7 autoantibody levels in sera from 38 gastric cancer patients and from control serum samples were also tested.

Results

The optimum conditions for recombinant MMP-7 protein expression were determined as 0.04 mmol/L Isopropyl-β-D-T

SUBMITTER: Zhou JH 

PROVIDER: S-EPMC3068276 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature