Examining the lateral displacement of HL60 cells rolling on asymmetric P-selectin patterns.

ABSTRACT: The lateral displacement of cells orthogonal to a flow stream by rolling on asymmetrical receptor patterns presents a new opportunity for the label-free separation and analysis of cells. Understanding the nature of cell rolling trajectories on such substrates is necessary to the engineering of substrates and the design of devices for cell separation and analysis. Here, we investigate the statistical nature of cell rolling and the effect of pattern geometry and flow shear stress on cell rolling trajectories using micrometer-scale patterns of biomolecular receptors with well-defined edges. Leukemic myeloid HL60 cells expressing the PSGL-1 ligand were allowed to flow across a field of patterned lines fabricated using microcontact printing and functionalized with the P-selectin receptor, lever

SUBMITTER: Lee CH 

PROVIDER: S-EPMC3068857 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature