The proteomics of quiescent and nonquiescent cell differentiation in yeast stationary-phase cultures.

ABSTRACT: As yeast cultures enter stationary phase in rich, glucose-based medium, differentiation of two major subpopulations of cells, termed quiescent and nonquiescent, is observed. Differences in mRNA abundance between exponentially growing and stationary-phase cultures and quiescent and nonquiescent cells are known, but little was known about protein abundance in these cells. To measure protein abundance in exponential and stationary-phase cultures, the yeast GFP-fusion library (4159 strains) was examined during exponential and stationary phases, using high-throughput flow cytometry (HyperCyt). Approximately 5% of proteins in the library showed twofold or greater changes in median fluorescence intensity (abundance) between the two conditions. We examined 38 strains exhibiting two distinct fluore

SUBMITTER: Davidson GS 

PROVIDER: S-EPMC3069023 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature