Consequences of the overexpression of a eukaryotic membrane protein, the human KDEL receptor, in Escherichia coli.

ABSTRACT: Escherichia coli is the most widely used host for producing membrane proteins. Thus far, to study the consequences of membrane protein overexpression in E. coli, we have focussed on prokaryotic membrane proteins as overexpression targets. Their overexpression results in the saturation of the Sec translocon, which is a protein-conducting channel in the cytoplasmic membrane that mediates both protein translocation and insertion. Saturation of the Sec translocon leads to (i) protein misfolding/aggregation in the cytoplasm, (ii) impaired respiration, and (iii) activation of the Arc response, which leads to inefficient ATP production and the formation of acetate. The overexpression yields of eukaryotic membrane proteins in E. coli are usually much lower than those of prokaryotic ones. This may

SUBMITTER: Klepsch MM 

PROVIDER: S-EPMC3069486 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature