Long-term magnetic resonance imaging of stem cells in neonatal ischemic injury.

ABSTRACT:

Objective

Quantitative magnetic resonance imaging (MRI) can serially and noninvasively assess the degree of injury in rat pup models of hypoxic ischemic injury (HII). It can also noninvasively monitor stem cell migration following iron oxide prelabeling. Reports have shown that neural stem cells (NSCs) may help mediate neuroprotection or stimulate neuroreparative responses in adult and neonatal models of ischemic injury. We investigated the ability of high-field MRI to monitor and noninvasively quantify the migration, proliferation, and location of iron oxide-labeled NSCs over very long time periods (58 weeks) in real time while contemporaneously correlating this activity with the evolving severity and extent of neural damage.

Methods

Labeled clonal murine NSCs (mNSCs) were

SUBMITTER: Obenaus A 

PROVIDER: S-EPMC3069664 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature