Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity.

ABSTRACT:

Background

Adipose tissue abundance relies partly on the factors that regulate adipogenesis, i.e. proliferation and differentiation of adipocytes. While components of the transcriptional program that initiates adipogenesis is well-known, the importance of microRNAs in adipogenesis is less well studied. We thus set out to investigate whether miRNAs would be actively modulated during adipogenesis and obesity.

Methods

Several models exist to study adipogenesis in vitro, of which the cell line 3T3-L1 is the most well known, albeit not the most physiologically appropriate. Thus, as an alternative, we produced EXIQON microarray of brown and white primary murine adipocytes (prior to and following differentiation) to yield global profiles of miRNAs.

Results

We found 65 miRNAs

SUBMITTER: Keller P 

PROVIDER: S-EPMC3070678 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature