Divergent mechanisms for passive pneumococcal resistance to β-lactam antibiotics in the presence of Haemophilus influenzae.

ABSTRACT:

Background

Otitis media, for which antibiotic treatment failure is increasingly common, is a leading pediatric public health problem.

Methods

In vitro and in vivo studies using the chinchilla model of otitis media were performed using a β-lactamase-producing strain of nontypeable Haemophilus influenzae (NTHi 86-028NP) and an isogenic mutant deficient in β-lactamase production (NTHi 86-028NP bla) to define the roles of biofilm formation and β-lactamase production in antibiotic resistance. Coinfection studies were done with Streptococcus pneumoniae to determine if NTHi provides passive protection by means of β-lactamase production, biofilm formation, or both.

Results

NTHi 86-028NP bla was resistant to amoxicillin killing in biofilm studies in vitro; however, it was clea

SUBMITTER: Weimer KE 

PROVIDER: S-EPMC3071235 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature