Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli.

ABSTRACT:

Background

Isobutanol is a promising next-generation biofuel with demonstrated high yield microbial production, but the toxicity of this molecule reduces fermentation volumetric productivity and final titer. Organic solvent tolerance is a complex, multigenic phenotype that has been recalcitrant to rational engineering approaches. We apply experimental evolution followed by genome resequencing and a gene expression study to elucidate genetic bases of adaptation to exogenous isobutanol stress.

Results

The adaptations acquired in our evolved lineages exhibit antagonistic pleiotropy between minimal and rich medium, and appear to be specific to the effects of longer chain alcohols. By examining genotypic adaptation in multiple independent lineages, we find evidence of parallel ev

SUBMITTER: Minty JJ 

PROVIDER: S-EPMC3071312 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature