Transcriptome analysis of female and male Xiphophorus maculatus Jp 163 A.

ABSTRACT:

Background

Xiphophorus models are important for melanoma, sex determination and differentiation, ovoviviparity and evolution. To gain a global view of the molecular mechanism(s) whereby gene expression may influence sexual dimorphism in Xiphophorus and to develop a database for future studies, we performed a large-scale transcriptome study.

Methodology/principal findings

The 454-FLX massively parallel DNA sequencing platform was employed to obtain 742,771 and 721,543 reads from 2 normalized cDNA libraries generated from whole adult female and male X. maculatus Jp 163 A, respectively. The reads assembled into 45,538 contigs (here, a "contig" is a set of contiguous sequences), of which, 11,918 shared homology to existing protein sequences. These numbers estimate that the conti

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC3071723 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature