Interaction and uptake of exosomes by ovarian cancer cells.

ABSTRACT:

Background

Exosomes consist of membrane vesicles that are secreted by several cell types, including tumors and have been found in biological fluids. Exosomes interact with other cells and may serve as vehicles for the transfer of protein and RNA among cells.

Methods

SKOV3 exosomes were labelled with carboxyfluorescein diacetate succinimidyl-ester and collected by ultracentrifugation. Uptake of these vesicles, under different conditions, by the same cells from where they originated was monitored by immunofluorescence microscopy and flow cytometry analysis. Lectin analysis was performed to investigate the glycosylation properties of proteins from exosomes and cellular extracts.

Results

In this work, the ovarian carcinoma SKOV3 cell line has been shown to internalize exo

SUBMITTER: Escrevente C 

PROVIDER: S-EPMC3072949 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature