A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability.

ABSTRACT:

Background

The production of cardiomyocytes from human induced pluripotent stem cells (hiPSC) holds great promise for patient-specific cardiotoxicity drug testing, disease modeling, and cardiac regeneration. However, existing protocols for the differentiation of hiPSC to the cardiac lineage are inefficient and highly variable. We describe a highly efficient system for differentiation of human embryonic stem cells (hESC) and hiPSC to the cardiac lineage. This system eliminated the variability in cardiac differentiation capacity of a variety of human pluripotent stem cells (hPSC), including hiPSC generated from CD34(+) cord blood using non-viral, non-integrating methods.

Methodology/principal findings

We systematically and rigorously optimized >45 experimental variables to dev

SUBMITTER: Burridge PW 

PROVIDER: S-EPMC3072973 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature