Heart failure-associated changes in RNA splicing of sarcomere genes.

ABSTRACT:

Background

Alternative mRNA splicing is an important mechanism for regulation of gene expression. Altered mRNA splicing occurs in association with several types of cancer, and a small number of disease-associated changes in splicing have been reported in heart disease. However, genome-wide approaches have not been used to study splicing changes in heart disease. We hypothesized that mRNA splicing is different in diseased hearts compared with control hearts.

Methods and results

We used the Affymetrix Exon array to globally evaluate mRNA splicing in left ventricular myocardial RNA from controls (n=15) and patients with ischemic cardiomyopathy (n=15). We observed a broad and significant decrease in mRNA splicing efficiency in heart failure, which affected some introns to a grea

SUBMITTER: Kong SW 

PROVIDER: S-EPMC3073230 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature